Fig. 3

CIITA inhibits Tat transactivating function in U937 Minus cells and in U937 Plus-CIITA clones. U937 Minus (black columns) and U937 Plus (gray columns) cells were co-transfected with fixed amount of pHIV-1 LTR-Luc, phRL-CMV, and increasing amounts (10, 20 ng) of plasmid coding for Tat (pcTat). CIITA-positive Plus clones 1C11, 1F6 and CIITA-negative Plus 4G2 clone were co-transfected with fixed amount of pHIV-1 LTR-Luc, phRL-CMV and 20 ng of pcTat. The basal HIV-1 LTR activity was set to 1 for each cell (−). Mean luciferase values, normalized to Renilla values, are presented as fold of activation (Tat-dependent vs basal). Results are the average of two independent experiments (**P < 0.005, unpaired two-tailed t test). Error bars show standard deviation