Fig. 3
From: Unexpected impairment of TNF-α-induced maturation of human dendritic cells in vitro by IL-4
IL-4 impairs the migration of TNF-α-matured DC towards CCL21, CCL19 and CCR7 expression. a, b The chemotactic migration of IL-4− and IL-4+-DC in response to rhCCL21 and rhCCL19 (500 or 1000 ng/mL) was assayed in vitro. Serum-free medium, either alone or with CCL21 or CCL19, was added to the wells, followed by 5 × 105 DC. For each experiment, non-specific migration in medium alone was deducted from specific migration in response to each chemokine. The data represent the mean ± SEM of migrated DC from five separate experiments. *P < 0.05, compared to IL-4−-DC. c IL-4− and IL-4+-DC were stained with PE-anti-CCR7 antibody (bold lines) or its isotype control (thin lines) and analyzed by flow cytometry. The results are representative of 11 independent experiments. d Mean fluorescence intensity (MFI) of surface CCR7 content expressed as the ratio of fluorescence intensity of specific labeling to background staining. The results are the mean ± SEM of the MFI of six independent experiments