Fig. 2

VEGF-B inhibited I/R-mediated cardiocyte apoptosis in vivo. a A typical image of cardiomyocyte apoptosis in the heart I/R model was detected and analyzed using the TUNNEL method. Red fluorescence apoptotic cells; Blue fluorescence cell nucleus. b Quantitative analysis of the cell apoptosis rate in HPF. *P < 0.01 vs. sham group; ^# P < 0.05 vs. I/R group, n = 6. c Bcl-2, pAkt, Bax, pERK1, pp38MAPK and LC3-II/I expression was detected using western blot, as described in the Materials and methods section (c), and quantified using normalization to a-tubulin (d–i). *P < 0.01 vs. sham group; ^# P < 0.05 vs. I/R group, n = 6