Fig. 3

Effects of Fn14-Fc on GC formation and B cell transcription factors in SLE mice. a Fn14-Fc or control-Fc-treated mice were sacrificed 21 days after the first injection. Spleens from the mice in each group (n = 5/group) were examined by immunofluorescence staining with monoclonal Abs against CD4 (white) and B220 (blue), GL-7 (green) and CD138 (red) (original magnification, ×400). b mRNA levels of AID, xbp-1, Blimp-1 and IRF4 in isolated splenocytes were determined by real-time PCR. Data are expressed as means ± SDs. *P < 0.05, **P < 0.01 compared to the control-Fc treated group