Fig. 1

TWEAK promotes B cell differentiation. a CD19⁺ B cells isolated from the spleens of sanroque mice were cultured with or without TWEAK (0.1 ng/ml). After 3 days, total RNA was extracted using the TRI reagent. The mRNA levels of various B cell differentiation markers—such as AID, Blimp-1, IRF4—were analyzed by real-time PCR. b Splenocytes, CD4⁺ T cells, and CD19⁺ B cells were isolated from the spleens of sanroque mice, total RNA was extracted using TRI reagent, and mRNA levels of Fn14 were analyzed by real-time PCR. c Spleens from the sanroque mice were examined by immunofluorescence staining with monoclonal Abs against B220 (blue) and Fn14 (red). Data are expressed as means ± SDs. *P < 0.05, ***P < 0.001 compared to the control group