Fig. 4

Detection of subpopulation of T cells and Treg cells of lymphocytes. Detection of subpopulation of T cells and Treg cells of lymphocytes exposed to the culture supernatant of SKOV3 cells treated with AO-1 alone or combined with LPS using FCM assay as described under “Methods” section. a FCM diagrams of CD3⁺/CD4⁺/CD8⁺ of T cells, and b FCM diagrams of CD4⁺CD25⁺FoxP3⁺ of Treg cells. All experiments were performed in triplicate, and data are expressed as mean ± SD (n = 3). Error bars represent SD of replicate data points. *P < 0.05, compared to the control