Fig. 2

Western blot detection for expression of involved proteins by EOC cells. a and b SKOV3 cells were treated with AO-1 alone or combined with LPS as indicated concentration for indicated time, and the expression of NF-κB p65, p-NF-κB p65, Akt and pAkt was determined by western blot as described under “Methods” section. c Represents IDO1 expression and IDO activity (l-kynurenine release). All experiments were performed in triplicate and data are expressed as mean ± SD (n = 3). Error bars represent SD of replicate data points. *P < 0.05, compared to the control