Fig. 6
Vemurafenib does not inhibit ex vivo maturation of pDCs and mDCs isolated from melanoma patients on active treatment. PBMCs were isolated from patients before and after 1 month on vemurafenib treatment. a The frequency of pDCs and mDCs in the PBMCs was measured by FACS analysis. b Freshly isolated PBMCs from melanoma patients after 1 month on vemurafenib treatment were cultured ex vivo and activated with R848. Graphs show the cell surface expression levels of CD80, CD86, PD-L1, MHC-I, and MHC-II on pDCs and mDCs after 18 h. c Freshly isolated PBMCs from melanoma patients after 1 month on vemurafenib treatment were stimulated with PHA and T cell proliferation was measured after 4 days with [3H] thymidine incorporation. Shown is the mean (+SEM) of seven independent experiments *P < 0.05, **P < 0.01, ***P < 0.001