Fig. 4
Vemurafenib impairs antigen presentation and allostimulatory capacity of ex vivo cultured pDCs and mDCs. Freshly isolated pDCs (a) and mDCs (b) were cultured ex vivo and activated with R848 in presence or absence of 60 μg/ml vemurafenib. Graphs show the cell surface expression levels of MHC class I and MHC class II after 18 h. Mixed lymphocyte reaction using freshly isolated, pDCs (c) and mDCs (d) stimulated with R848 in presence or absence of 60 μg/ml vemurafenib. After maturation DCs were incubated with allogeneic PBLs and T cell proliferation was measured after 4 days with [3H] thymidine incorporation. d pDCs and mDCs from a HLA-A2.1 + donor were loaded with different concentrations of a melanoma-specific peptide (gp100280:288) or irrelevant peptide (tyrosinase369:376) in the presence of R848 with/without vemurafenib and gp100280:288 specific T cells. Graphs show the cell surface expression levels of the early activation marker CD69 after overnight co-culture. Shown is the mean (+SEM) of at least three independent experiments *P < 0.05, **P < 0.01, ***P < 0.001