Fig. 3

Dabrafenib and trametinib do not impair maturation of ex vivo cultured pDCs and mDCs. Freshly isolated pDCs (a) and mDCs (b) were cultured ex vivo and activated with R848 in presence or absence of 60 μg/ml vemurafenib, 90 ng/ml dabrafenib and 12 ng/ml trametinib. Graphs show the cell surface expression levels of CD80, CD86, PD-L1, MHC-I and MHC-II after 18 h. Shown is the mean (+SEM) of three independent experiments *P < 0.05, **P < 0.01,***P < 0.001