Fig. 2

RCC-reactive CD8⁺ T cells from patient TC exhibit reduced levels of Jak3 and MCL-1 proteins. Representative immunoblots are provided for d35-cultured CD8⁺ T cells isolated from patient TC versus normal donors using specific antibodies against total Jak3 (a) and MCL-1 (c) protein. Control antibodies recognizing actin are also provided. Relative levels of Jak3 and MCL-1 (both normalized to actin) are presented in panels b and d, respectively. Results in panels b and d are reported as the mean (±SD) of ratios obtained in three independent experiments. p < 0.005 for RCC vs donor 2; p < 0.0005 for RCC vs donor 3