Fig. 5

Determination lipid peroxidation by 4-HNE-fluorescence analysis and poly-ubiquitination in WT and Nrf2^−/− mice. a IF analysis using anti-4HNE-ab (1:500; v/v) showing increased HNE adducts in WT and Nrf2^–/– mice. Blue: nucleus (DAPI); Green: Anti-4HNE staining. b Anti-4HNE intensity was calculated as fold change of mean values of the WT sedentary group and the data is represented as mean ± SEM in the histograms (n = 3–4/group). c IF of poly-ubiquitination in WT and Nrf2^−/− mice. Images were obtained under confocal microscope at a magnification of 60x oil immersion. Blue: nucleus (DAPI); Green: Ubiquitinated proteins. d Intensity analysis of ubiquitinated proteins showing ~ fivefold increase in Nrf2^−/− subjected HIES when compared to WT HIES or WT-sedentary mice (n = 3–4/group). *P < 0.05 vs SED WT; ^#P < 0.05 vs SED Nrf2^–/– and ^$P < 0.05 vs HIES WT