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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: IL-17 mediates inflammatory reactions via p38/c-Fos and JNK/c-Jun activation in an AP-1-dependent manner in human nucleus pulposus cells

Fig. 6

Regulation of COX2 and PGE2 expression is dependent on p38/c-fos and JNK/c-Jun activities. a Effects of IL-17A and MAPK inhibitors on IL-17A induced c-Jun and c-Fos translocation. Nucleoprotein was isolated from NP cells treated with SB203580 (10 or 20 μM), or SP600125 (10 or 20 μM) for 30 min prior to IL-17A (100 ng/ml) induction for 24 h. Afterward, western blot analysis was performed using specific antibodies against c-Jun and c-Fos. Expression of lamin B1 was examined as a reference. The upper panel suggested that the JNK inhibitor SP600125 effectively inhibited IL-17A-induced c-Jun translocation, while the lower panel showed that the p38 inhibitor SB203580 significantly inhibited IL-17A-induced c-Fos translocation. b Pretreatment with combination of SB203580 (10 or 20 μM) and SP600125 (10 or 20 μM) for 30 min prior to IL-17A induction for 24 h in NP cells significantly declined the expression of COX2 and PGE2 (*P < 0.01, vs. control; #P < 0.001, vs. IL-17-only treatment). c IL-17A-stimulated COX2 and PGE2 expression depends on AP-1 activation. Pretreatment with curcumin (30 μM) for 30 min prior to IL-17A (100 ng/ml) induction for 24 h in NP cells significantly reduced the expression of COX2 and PGE2 compared with IL-17A-only treatment. (*P < 0.01, vs. control; #P < 0.001, vs. IL-17-only treatment)

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