Fig. 5

IL-17A induces phosphorylation of c-Jun in NP cells. Primary NP cells were transferred to a 24-well plate and cultured in serum-free medium for 12 h before treatment with SP600125 (20 μM; JNK MAPK phosphorylation inhibitor) for 30 min prior to the addition of IL-17A (100 ng/ml) for 4 h. The cells were performed to Immunofluorescence staining. NP cells were incubated with primary antibodies against p-c-jun and anti-rabbit IgG secondary antibody (FITC). DAPI mounting medium was used for nuclear staining. Left: cells stained with antibody to p-c-Jun. Middle: cells stained with DAPI to identify nuclei, (blue). Right: cells stained with antibody to p-c-Jun and with DAPI. The phosphorylation level of c-Jun was promoted strongly in IL-17A treated NP cells than in untrated controls, and the phosphorylation induced by IL-17A can be attenuated by JNK inhibitor SP600125 (20 μM). Scale bar = 50 μm