Fig. 1

Effect of IL-17A on expression of COX2 and PGE2 in NP cells. a IL-17A induced COX2 expression in a concentration-dependent manner. NP cells were stimulated with the indicated concentrations of IL-17A for 24 h. Western blot analysis demonstrated that IL-17A of 10 ng/ml could significantly increase the COX2 expression and the optimal concentration was 100 ng/ml. b IL-17A induced COX2 expression in a time-dependent manner. Cell cultures were treated with optimal concentration of IL-17A for 0, 6, 12 and 24 h. Western blot results showed that IL-17A (100 ng/ml) could induce a time-dependent increase of COX2. c ELISA analysis revealed that IL-17A induced PGE2 secretion in a dose-dependent manner. The cell-free supernatants were harvested and the expression levels of PGE2 protein were quantified by ELISA. d IL-17A induced PGE2 secretion in a time-dependent manner. Regarding to both COX2 and PGE2, there were statistically significant differences between concentrations higher than 10 ng/ml and the control. And, statistical significance was verified of IL-17A treatment longer 12 h. (a and c: *P < 0.01, vs. control; ^#P < 0.001, vs. 10 ng/ml; b and d: *P < 0.01, vs. control; ^#P < 0.001, vs. 12 h.)