Fig. 6

Regulation of the expression of B2M at mRNA and protein levels by the TGF-β signaling pathway in ovarian cancer cell lines. OVCAR-3 and SK-OV-3 cells were pre-treated with a TGF-β type I receptor kinases inhibitor (SB431542, 10 μM) for 30 min and then treated with 10 ng/ml TGF-β1. B2M mRNA in OVCAR-3 (a) and SK-OV-3 (b) cells was detected by quantitative RT-PCR using primers specific to B2M after TGF-β1 treatment for 24 h. B2M protein in OVCAR-3 (c) and SK-OV-3 (d) cells was detected by Western blot analysis using a specific antibody against B2M and β-actin after TGF-β1 treatment for 72 and 48 h, respectively. Histograms show the quantitative analysis of the gels after densitometry. The results are representative of three independent experiments. *P < 0.05