Fig. 3
Effect of Hericium erinaceus mycelium (HEM) on brain histological nitrotyrosine and 4-HNE protein expression in MPTP animal model. Histological examination of brain were revealed to somata of dopamine neurons by immunohistochemical staining. a Mice were sacrificed and the brains were separated. Representative brain sections stained control group (I); Mice treated with MPTP at 6 days (I); HEM administration [10.76 (III) and 21.52 (IV) mg/day] staining of nitrotyrosine (a) and 4-HNE (b) protein in brain tissue. c Evaluation of nuclear protein expression and nitrotyrosine and 4-HNE expression were quantitative in the striatum. The area of dopamine neurons positive stained area from the SN was evaluated from three randomly selected observation fields of each brain section. Quantitative of immunohistochemical proteins by average integrated optical density (AIOD). Positive stained area was evaluated from three randomly selected observation fields of each liver section. Data were expressed as mean ± SD (n = 6/group). *p < 0.05, nitrotyrosine expression, MPTP group versus MPTP + HEM group.# P < 0.05, 4-HNE expression, MPTP group versus MPTP + HEM group