Fig. 2

Effect of Hericium erinaceus mycelium (HEM) on brain histological examination in MPTP treated animal model. MPTP animal models (MPTP injection and brains harvested) were performed on mice described in “Methods” section. a Histological examination of brain were revealed to striatum and substantia nigra zones as indicated by immunohistochemical staining of TUNEL. Representative brain sections stained as saline infusion control group (I); Mice with MPTP injection (II); HEM treatment (10.76 and 21.52 mg/day) (III, IV); Apoptotic cells were measured under microscopy as described in “Methods” section. The MPTP group exhibited dopamine deficiency syndrome of the striatum, as indicated by apoptotic cells (filled arrow). Magnification, ×100. b Histological examination of brain were revealed in brain, as indicated by immunohistochemical staining of tyrosine Hydroxylase p-Ser 31. Magnification, ×200. The cells were counted from 10 fields (200× magification) of each brain sample. c The results from statistical analysis are the means of cells and were calculated per microscope field from six animals per group. Data are expressed as mean ± SD of independent experiments. *p < 0.05, MPTP group versus MPTP + HEM group. ^# P < 0.05, MPTP group versus MPTP + HEM group