Fig. 2

Expression of PRL-3 in prostate cancer cell lines PC3 and DU145, which have an increased copy number of PRL-3 gene locus. a Prostate cancer cell lines PC3 and DU145 were analyzed for PRL-3 expression by quantitative RT-PCR. PRL-3 transcript levels were normalized to GAPDH expression in each sample and presented as relative to the PRL-3 expression in the PC3 cell line, which was set to one. Mean CT values are presented in the bars. b PRL-3 protein expression was determined by Western blotting. The INA-6 cell line was used as positive control. GAPDH was used as loading control. c, d Fluorescence in situ hybridization with whole chromosome and PRL-3 probe on prostate cancer cell lines PC3 (c) and DU145 (d). The red signal marks PRL-3 (8q24.3) and the green signal marks the whole chromosome 8