Gene expression analysis of bone metastasis and circulating tumor cells from metastatic castrate-resistant prostate cancer patients

Table 3 Yield of aRNA after one and two rounds of amplification of RNA obtained from five cultured prostate cancer cells

Cell line	Median (range) total RNA/5 cells (pg)^a	Median (range) mRNA/5 cells (pg)^b	Median (range) yield of aRNA/5 cells (ng)
PC3	194.3 (150–233)	5.83 (4.5–6.9)	495 (94.4–630) (1st round)
PC3	194.3 (150–233)	5.83 (4.5–6.9)	4650 (1200–7200) (2nd round)
LNCaP	130.0 (105–155)	3.9 (3.2–4.7)	525 (310–680) (1st round)
LNCaP	130.0 (105–155)	3.9 (3.2–4.7)	4950 (1500–6300) (2nd round)

ISSN: 1479-5876