Fig. 3

Gene profiles shown by amplified aRNA obtained from CTCs and PCa tissue microdissected from FFPE BMBx. a RT-PCR analysis of aRNA amplified from CTCs. b Representative section obtained from a FFPE BMBx of patient 24R-G, with PCa metastatic to bone, as confirmed by pan-CK immunostaining (top left picture). An adjacent section stained with H&E (top right) was used to microdissect (bottom left), and capture (bottom right) areas containing PCa cells only. Bar magnification, 200 μm. c RT-PCR analysis of aRNA amplified from PCa microdissected from FFPE BMBx from four mCRPC patients