Fig. 2

Quality of aRNA amplified from CTCs and LCM bone metastases from mCRPC patients. a Primers designed to amplify 5′-end, middle (M), and 3′-end fragments of EpCAM and GAPDH transcripts. b RT-PCR products obtained for amplified aRNA derived from CTCs and laser microdissected tumor cells metastatic to bone of three mCRPC patients, using the two sets of primers targeting different regions of EpCAM and GAPDH