Fig. 1
Comparison of gene transcript levels in total RNA and amplified aRNA. Transcriptional profiles assessed by RT-qPCR for genes with high (EpCAM), intermediate (SNAI2, ZEB1), and low (BRCA2) expression levels in total RNA from high number of cultured PC3 cells, and amplified aRNA from five PC3 cells spiked in blood after CellSearch® Profile kit processing. Relative expression of genes was normalized to that of GAPDH, calculated by the delta–delta-CT method. No significant statistical differences in relative expression for each particular transcript were observed among non-amplified mRNA, one-round amplified aRNA, and two-round amplified aRNA, as per analysis of variance (ANOVA)