Fig. 9

Fluorochrome-labeling of new bone formation and mineralization in the defects. a Column 1 (yellow) shows the deposition of tetracycline at week 2, column 2 (red) shows the deposition of alizarin red at week 4, column 3 (green) shows the deposition of calcein at week 6, column 4 represents the merged images of the three fluorochromes for the same group, and column 5 represents the merged images of the three fluorochromes with a plain CLSM image for the same group; b the percentages of three fluorochrome areas in the defects. Bars represent the means and standard deviation (n = 10); * indicates the statistically significant difference compared with the control group (P < 0.05); ^# indicates the statistically significant difference compared with the L-PRP group (P < 0.05)