Fig. 4

SiRNA-mediated HMGA1 knockdown inhibited thyroid cancer cell proliferation and invasion. a Growth curves of cells in each group. MTT analysis showed that siRNA-mediated HMGA1 knockdown significantly inhibited thyroid cancer TPC1 cell proliferation (n = 3). *P < 0.05. b Colony formation assay showed that siRNA-mediated HMGA1 knockdown significantly inhibited thyroid cancer TPC1 cell colony formation (n = 3, **P < 0.01). c RT-PCR and western blot assay showed that siRNA-mediated HMGA1 knockdown inhibited mRNA and protein expression of HMGA1 and Snail, promoted mRNA and protein expression of E-cadherin in thyroid cancer SW579 cell. d Transwell invasion assay showed that siRNA-mediated HMGA1 knockdown significantly inhibited thyroid cancer SW579 cell invasion. Cells were transiently transfected with HMGA1-siRNA and plated on the top of the transwells. Twenty-four hours after plating, cells invaded through the pores were counted. Values are expressed as mean ± SD of three independent experiments, **P < 0.01