Fig. 1

Effects of Bortezomib on proliferation, cell cycle progression, and apoptosis in CML cells. a Bortezomib inhibits the cell viability of CML cells. AR230, LAMA-84 and K562 cells were incubated with 10, 25, 50 and 100 nm bortezomib for 24 h. Cell proliferation assays were performed using MTT as described in “Methods” section. The graph displays the mean ± SD (standard deviation) of three independent experiments with replicates of six wells for all the doses. **p < 0.01, ***p < 0.001 b Bortezomib induces the increase of subG0 population of CML cells. K562 and AR230 cells were treated with 10, 25 and 50 nm of bortezomib for 24 h. Thereafter, the cells were washed, fixed and stained with propidium iodide, and analyzed for DNA content by flow cytometry as described in “Methods” section. c Bortezomib induces apoptosis in CML cells. K562 and AR230 cells were treated with 10, 25 and 50 nm of bortezomib for 24 h and cells were subsequently stained with flourescein-conjugated annexin-V and propidium iodide (PI) and analyzed by flow cytometry. d Bortezomib treatment of CML cells induces DNA fragmentation. K562 and AR230 cells were treated with 10, 25 and 50 nm bortezomib as indicated for 24 h and DNA was extracted and separated by electrophoresis on 1.5 % agarose gel