Fig. 4

Levels of degranulating CMV-specific CD8⁺ T cells in AAD patient and control cells. PBMC from AAD patients and healthy controls were stimulated with HLA-specific CMV peptide (1 µg/mL) and expanded for 13 days, before being re-stimulated with CMV peptide and stained with anti-human CD107a for 4 h. The cells were also stained with anti-human CD8 before being analyzed on an Accuri C6 flow cytometer. Results are expressed as percentage of CD8⁺ T cells positive for CD107a in patients and controls (a). The same data are also shown grouped into HLA-B8 (b) and HLA-A2 (c) positive patients (b n = 3, c n = 4) and controls (b n = 7, c n = 3). Non-parametric Mann–Whitney U test was used to test for statistical differences between patients and controls, but none were found. The bars display the mean for the whole group