Fig. 1
Baseline S. Typhi-specific CD8+ T cell responses define the clinical outcome after challenge. PBMC isolated at baseline from each participant (TD n = 7, blue; NoTD n = 9, red) were stimulated for 18 h with S. Typhi-infected AEH cells (squares), S. Typhi-infected B-EBV cells (circles) or S. Typhi-infected blasts (triangles). After co-culture, cells were immunostained with a 14-color panel of mAbs and analyzed by follow cytometry as described in Methods. Each symbol represents the net percentage of positive cells measured for CD107a, IFN-γ, TNF-α, MIP-1β, IL-17A and IL-2 in the CD8+ TEM, TEMRA and TCM subsets as indicated for each participant. Horizontal lines represent the median for each group. Statistical analyses were performed using mixed effects models to account for multiple observations per person. *p < 0.05, **p < 0.01