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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Combination of double negative T cells and anti-thymocyte serum reverses type 1 diabetes in NOD mice

Fig. 1

DN T cells showed different immune regulation of CD4+ and CD8+ T cells in vitro and in vivo. a DN T cells potently suppressed CFSE-labeled CD4+ and CD8+ T cell proliferation triggered by mDCs in vitro. The horizontal bars gate the un-dividing cells, and the numbers refer to the percentages these cells comprise of the total CD4+ or CD8+ T cells respectively. b The data are shown as percent inhibition of proliferation compared with controls, to which no DN T cells were added. The results reported are representative of three experiments with similar results. c The rejection of a skin graft from DBA/2 mice transplanted to C57BL/6 RAG−/− mice was induced by adoptive transfer of naïve C57BL/6 CD4+CD25 T cells or CD8+ T cells. C57BL/6 DN T cells were co-transferred by tail vein injection. Graft survival was observed by daily visual inspection. DN T cells suppressed naïve CD4+CD25 T cell-triggered skin allograft rejection. d DN T cells failed to prolong naïve CD8+ T cell-triggered skin allograft rejection. Statistical analysis was performed using a log-rank test

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