Fig. 5
From: Exosome-mediated transfer of miR-222 is sufficient to increase tumor malignancy in melanoma
Effects on melanoma tumorigenesis of antagomir-221&222 carried by EXOs. Melanoma cells treated with control antagomir-133 (αmiR-133)- or antagomir-221&222 (αmiR-221 + 222)-EXOs were compared for a modulation of protein expression by western blot, b cell cycle rate, c tube-like formation capability evaluated at 20 h and 2 days after EXO internalization. qRT-PCR evaluation of d miR-222 related molecules and e miR-222 itself to confirm its inhibition. Relative miR expression levels were normalized on RNU6B. Data are representative of two independent experiments. *p < 0.05; **p < 0.01. β-Actin and GAPDH were utilized as internal controls