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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: Exosome-mediated transfer of miR-222 is sufficient to increase tumor malignancy in melanoma

Fig. 2

Characterization of melanoma exosomes. a Representative qRT-PCR in miR-222- and empty vector-transduced melanoma cells and corresponding exosomes (EXO EQ). Relative miR expression levels were normalized on miR-16 (for EXOs) or RNU6B (for cells). b, c Size distribution of Me1402/R UC and EQ purified vesicles analyzed by the Nanosight™ technology. Graph bars represent the mean size of the particles ±SD, whereas the mode, shown as a single point overlying the graphs, indicates the most often occurring dimension. d The relative amounts of melanoma-derived EXOs were assessed by using the Nanosight™ technology (left) or by the Bradford assay for protein quantization (right). e WB analysis of specific ‘‘exosome-enriched’’ proteins in EXOs purified from Me1007 and Me1402/R melanomas by the EQ method. β-Actin was utilized as internal loading control for each experiment. Columns, mean ± SD of at least three independent experiments. *p < 0.05; **p < 0.01

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