Fig. 4

IL2-GMCSF enhanced cytotoxicity in vitro. Cytotoxicity was measured using the WST-8 method to detect the viability of remained target cells. a–b Effects of E:T ratio of splenoctyes and B16F10 cells (a) and IL2-GMCSF dosages (b) were determined firstly. Accordingly, cytotoxicity of splenocytes against B16F10 cells (c), B16F10-GMCSF (d), RAW264.7 (e) and A1.1 (f) at the 20:1 E:T ratio in the presence of various IL2-GMCSF dosages demonstrated the promotion in cytotoxicity by IL2-GMCSF. *P < 0.05 compared with the control without any cytokine. These experiments were repeated at least three times with similar results