Fig. 4

Cells survived drug treatments in stiff gel conductions are still capable of forming tumoriods in vitro and xenograft tumor in vivo. a A schematic illustration of the experimental procedure; b surviving cells have similar proliferation potentials when placed in competent conditions; Doubling time of survived MDA-MB-231 and CF-PAC-1 were similar compared to non treated control; c both drugs treated MDA-MB-231 cells were able to develop mature tumor in vivo after 4-week of inoculation in athymic nude mice (n = 6); Blue arrows showing angiogenesis occurrence in the tumors and white arrow revealing tumor cell muscle infiltration