Fig. 3

Inhibition of cell growth by o-PDA and cyclophosphamide. MDA-MB-231 cells (1.25 × 10⁴ cells/well) were incubated over various time periods in the presence of cyclophosphamide alone (10 mM) (), o-PDA alone (32 μΜ) (), or the combination of the two reagents () for up to 48 h. Cells were incubated with Puromycin (25 mM) for 48 h (). Cell growth was evaluated by colorimetric assays using WST- as an indicator. Experiments were repeated three times. Results were demonstrated as a mean ± SD of OD₄₅₀. *p < 0.001 (calculated by Student’s two-tailed t test)