Fig. 5
From: c-Jun N-terminal kinase activation by nitrobenzoxadiazoles leads to late-stage autophagy inhibition
Cell death studies in JNK1-silenced cells. a Caspase-3 activity assay in scrambled shRNA- and JNK1 shRNA-transfected cells treated with vehicle (CTRL), 5 μM MC3181 or 5 μM NBDHEX for 24–48 h (mean ± SEM, n ≥ 3; *p < 0.05 vs. CTRL). b, c Analysis of DNA content by flow cytometry on scrambled shRNA- and JNK1 shRNA-transfected cells treated with vehicle (CTRL), 5 μM MC3181 or 5 μM NBDHEX for 48 h. b A representative experiment with the percentage of sub-G1 apoptotic cells is shown. c Histograms illustrating the percentage of cells in each phase of cell cycle, resulting from four independent experiments