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Table 2 Histological study of the graft at various time points

From: Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation

Condition Time points Histology Immunolabeling
Tubular lesions Lymphocyte infiltration Fibrosis and tubular atrophy Caspase-3 (%) CD10
Air-Celsior Removal 2.2 ± 0.1 1 1 3 +++
End of storage 2.3 ± 0.1 1 1 5 +++
Autopsy 4.7 ± 0.2 1.8 ± 0.2 2.7 ± 0.1 40 +
Argon-Celsior Removal 2.1 ± 0.1 1 1 3 +++
End of storage 2.2 ± 0.1 1 1 5 +++
Autopsy 3.3 ± 0.1* 1.2 ± 0.1 1.3 ± 0.1* 24 +++
Xenon-Celsior Removal 2.0 ± 0.1 1 1 4 +++
End of storage 2.2 ± 0.1 1 1 6 +++
Autopsy 4.6 ± 0.1 1.9 ± 0.1 2.6 ± 0.2 0
  1. Tubular lesions (1: no lesions; 2: lesions affecting <10 % of the sample; 3: 10–25 %; 4: 26–50 %; 5: >50 %), lymphocyte infiltration (1: no infiltrate; 2: loose, thin, dispersed infiltrate; 3: more abundant infiltrate, occasionally nodular; 4: dense, diffuse infiltrate with confluence), and fibrosis and tubular atrophy were scored (1: no fibrosis; 2: minimal fibrosis; 3: organized fibrosis with few glomerulosclerosis and tubular atrophy <25 %; 4: mutilating fibrosis with serious glomerulosclerosis and tubular atrophy >50 %). Data are means ± SDs; p < 0.050 (*): Argon-Celsior vs. Air-Celsior. Caspase-3 labeling was given as the percentage of cells expressing the antigen. CD10-labeling was determined using a semi-quantitative visual grading system (−: no labeling; +: weak; ++: moderate; +++: strong)