|
Condition
|
Time points
|
Histology
|
Immunolabeling
|
|---|
|
Tubular lesions
|
Lymphocyte infiltration
|
Fibrosis and tubular atrophy
|
Caspase-3 (%)
|
CD10
|
|---|
|
Air-Celsior
|
Removal
|
2.2 ± 0.1
|
1
|
1
|
3
|
+++
|
|
End of storage
|
2.3 ± 0.1
|
1
|
1
|
5
|
+++
|
|
Autopsy
|
4.7 ± 0.2
|
1.8 ± 0.2
|
2.7 ± 0.1
|
40
|
+
|
|
Argon-Celsior
|
Removal
|
2.1 ± 0.1
|
1
|
1
|
3
|
+++
|
|
End of storage
|
2.2 ± 0.1
|
1
|
1
|
5
|
+++
|
|
Autopsy
|
3.3 ± 0.1*
|
1.2 ± 0.1
|
1.3 ± 0.1*
|
24
|
+++
|
|
Xenon-Celsior
|
Removal
|
2.0 ± 0.1
|
1
|
1
|
4
|
+++
|
|
End of storage
|
2.2 ± 0.1
|
1
|
1
|
6
|
+++
|
|
Autopsy
|
4.6 ± 0.1
|
1.9 ± 0.1
|
2.6 ± 0.2
|
0
|
−
|
- Tubular lesions (1: no lesions; 2: lesions affecting <10 % of the sample; 3: 10–25 %; 4: 26–50 %; 5: >50 %), lymphocyte infiltration (1: no infiltrate; 2: loose, thin, dispersed infiltrate; 3: more abundant infiltrate, occasionally nodular; 4: dense, diffuse infiltrate with confluence), and fibrosis and tubular atrophy were scored (1: no fibrosis; 2: minimal fibrosis; 3: organized fibrosis with few glomerulosclerosis and tubular atrophy <25 %; 4: mutilating fibrosis with serious glomerulosclerosis and tubular atrophy >50 %). Data are means ± SDs; p < 0.050 (*): Argon-Celsior vs. Air-Celsior. Caspase-3 labeling was given as the percentage of cells expressing the antigen. CD10-labeling was determined using a semi-quantitative visual grading system (−: no labeling; +: weak; ++: moderate; +++: strong)
