Fig. 3

Hemin-induced activation and inactivation of Nrf2 and NF-kB p65 in lung, respectively, in rats with OALT. Immunofluorescent staining of Nrf2 (a, b) and NF-κB P65 (a, c) using lung tissue sections of groups sham, OALT + saline, OALT + Hemin and OALT + ZnPP groups at 8 h after reperfusion. Rats, intraperitoneally injected with saline, hemin (30 mg/kg) and ZnPP (20 mg/kg) separately in corresponding groups 24 h before operation, were received celiotomy and vascular separation with or without OALT. The results were expressed as the mean ± SD, n = 8 per group. *P < 0.05, **P < 0.01, one-way ANOVA with Tukey test