Fig. 7

MSC and MSC-CM down-regulate macrophages and converts the CD4⁺ T lymphocyte system into an anti-inflammatory state in CCl₄-stimulated spleens. Both MSC and MSC-CM treatment had no significant effect on (a) Th1. A significant reduction of (b) Th17 was observed 3 weeks after MSC-CM but not MSC treatment. However, (c) an increase in Th2 and (e) a reduction in macrophages was observed after both MSC and MSC-CM treatment. MSC-CM but not MSC treatment up-regulated (d) Treg. Quantification of (f) Th1, (g) Th17, (h) Th2, (i) Treg, and (j) macrophages in the spleen. k Spleen weight in MSC-CM-treated mice with chronic liver fibrosis. l Spleens from control and MSC-CM-treated CCl₄-stimulated mice