Fig. 1

CD24 is associated with EGFR expression in gastric cancer cells. a SGC-7901 cells were transfected with negative control siRNA or siRNA specifically targeting CD24 (siCD24). Forty-eight h later, total protein extracts from cells were analyzed by western blotting analysis for CD24 and EGFR expression. Western blot bands corresponding to EGFR and CD24 were quantified and normalized against GAPDH. Asterisk P < 0.05 in the siCD24 cells relative to siRNA control cells. b qPCR and c Western blotting assays of CD24 mRNA and protein expressions in GES-1, SGC-7901 and BGC-823 cells. Data in (b) are presented as mean ± SD of 3 determinations asterisk P < 0.05 in the cultures of BGC-823, SGC-7901 relative to the cultures of GES-1. d BGC-823 cells transfected with control siRNA or siCD24 were lysed, EGFR and CD24 level was determined by Western blotting assays. e GES-1 cells were transfected with empty vector or CD24 plasmids, and the total cellular proteins were extracted and analyzed for expressions of EGFR by western blotting assays