Table 1 Primer pairs of RT-PCR experiment
Gene name | NCBI reference sequence | Primer sequence (forward 5′–3′) | Primer sequence (reverse 5′–3′) | Product size (bp) |
|---|---|---|---|---|
nanog | XM_002712762.1 | GCCAGTCGTGGAGTAACCAT | TGTGCTGTGTTCTGGCTTTC | 196 |
oct3/4 (Pou5f1) | NM_001099957.1 | GAGATTTGCAAAGCGGAGAC | CGGTTACAGAACCACACA CG | 188 |
CD44 | FJ360436.1 | CCACGTGGAGAAAAATGGTC- | CACGTGCCCTTCTATGAACC | 157 |
klf4 | XM_008255400.1 | TTCAACGACCTTCCTGAACC | TCGGGGTAACCTGAAAACTG | 184 |
Gapdh | NM_001082253.1 | AGGTCATCCACGACCACTTC | GTGAGTTTCCCGTTCAGC C | 202 |
Nestin | XM_008264301.1 | GTGAGTTTCCCGTTCAGC C | CTCCACATCAGAAGCAGCAA | 176 |
Aldh1a1 | AY038801.1 | CTACCATCAGGGCCAGTGTT | CCCCTTCTTTCTTCCCACTC | 209 |