Fig. 3From: Detection of ALDH1 activity in rabbit hepatic VX2 tumors and isolation of ALDH1 positive cancer stem cellsAnalysis of mRNA expression. RNA were extracted from VX2 tumors and from normal rabbit liver cells as a control. a Semi-quantitative RT-PCR expression analysis revealed that VX2 tumors have higher levels of cancer stemness markers (nestin, klf4, nanog, oct3/4, Nanog,CD44 and Aldh1a1) in comparison to normal liver cells. b Real-time PCR using Ssofast Evagreen demonstrated similar trend of mRNA expression in VX2 tumors and in normal liver cells. Gapdh was used us controlBack to article page