Fig. 4

Flow cytometric and affinity chromatography analyses of cellular and humoral peritoneal immunity in CpG-ODN1826 (20 µg/mouse, qdx5d/wx4w) treated late-stage STO tumor-bearing mice. a Percentage of NK cells (CD49b+), monocytes/macrophage precursors (CD11b+ F4/80low), DC (CD11b+ CD11c+), granulocytes (CD11b+ Ly6G+), macrophages (CD11b+ F4/80high) among CD45 + cells and dot plots of significantly modulated subpopulations. b Percentage and dot plots of B-1 cells (CD19 + gated on CD11b+ CD23- among CD45 + FSClowSSClow cells) expressing or not CD5 (left); IgM concentration (right). All histograms represent pooled data (mean ± SE) from peritoneal lavages of 4 mice/group. *P < 0.05; **P < 0.01; ***P < 0.001