Fig. 5From: Overexpression of HMGA2 promotes tongue cancer metastasis through EMT pathwayKnockdown of HMGA2 can reversed epithelial–mesenchymal transition (EMT). a The expression of EMT markers of E-Cadherin, Vimentin, N-Cadherin and Snail were analyzed by western blotting both in Cal27 and UM1 tongue cancer cells. GAPDH was probed as the loading control. b Immunofluorescence staining analysis EMT markers of E-Cadherin and Vimentin (red) using confocal and the nuclei were stained with DAPI (blue) (scale bar: 5 μm)Back to article page