Fig. 2

HMGA2 is upregulated in histopathological sections of tongue cancer and high expression of HMGA2 is correlated with tumorigenesis and metastasis. a Western blotting analysis of HMGA2 protein in eight human primary tongue cancer (T) and paired adjacent non-tumor tongue tissues (N), with each pair taken from a same patient. b Quantitative real time RT-PCR analysis of HMGA2 mRNA from the same eight pairs of tongue cancer and adjacent non-tumor tongue tissues. Error bars represent SDs calculated from three parallel experiments. c HMGA2 expression levels were up-regulated in primary tongue tumor tissues (T) compared to the paired adjacent non-tumor tongue tissue (N) from the same patient as examined by immunohistochemistry. d Expression levels of HMGA2 in 20 paired TSCC and adjacent non-tumor tongue tissues. Alteration of expression is shown as box plot presentations and the mean level of HMGA2 expression in TSCCs were significantly higher than that in non-tumor tissues. (P < 0.001, independent t test). e Expression levels of HMGA2 between 60 TSCCs with and without metastasis. The mean level of HMGA2 expression in TSCCs with metastasis were significantly higher than that in TSCCs without metastasis (P < 0.001, independent t test)