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Table 2 The primers of target sequences

From: Upregulation of miR-483-3p contributes to endothelial progenitor cells dysfunction in deep vein thrombosis patients via SRF

Gene name

Primer usage

Sequence (5′–3′)

hsa-miR-199a-3p

RT primer

GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCTGCCC

Forward primer

GGTGTCACTCCTCTCCTCC

Reverse primer

CAGTGCGTGTCGTGGA

hsa-let-7e-5p

RT primer

GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAACTAT

Forward primer

GGGGTGAGGTAGGAGGTTGT

Reverse primer

GTGCGTGTCGTGGAGTCG

hsa-miR-483-3p

RT primer

GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAAGACG

Forward primer

GGTGTCACTCCTCTCCTCC

Reverse primer

CAGTGCGTGTCGTGGA

U6 (human)

RT primer

CGCTTCACGAATTTGCGTGTCAT

Forward primer

GCTTCGGCAGCACATATACTAAAAT

Reverse primer

CGCTTCACGAATTTGCGTGTCAT

hsa-miR-199a-3p

Agomir: sense

ACAGUAGUCUGCACAUUGGUUA

Agomir: antisense

ACCAAUGUGCAGACUACUGUUU

Antagomir: sense

UAACCAAUGUGCAGACUACUGU

hsa-let-7e-5p

Agomir: sense

UGAGGUAGGAGGUUGUAUAGUU

Agomir: antisense

CUAUACAACCUCCUACCUCAUU

Antagomir: sense

AACUAUACAACCUCCUACCUCA

hsa-miR-483-3p

Agomir: sense

UCACUCCUCUCCUCCCGUCUU

Agomir: antisense

GACGGGAGGAGAGGAGUGAUU

Antagomir: sense

AAGACGGGAGGAGAGGAGUGA

rno-miR-483-3p

Overexpression

GGAUCCGCACUCCUCCCCUCCCGUCUUGUCUGAGAAACAAGACGGAAGGAAACGUCACUUUUUUGAAUUC

rno-miR-483-3p sponge

Sponge

GGAUCCACAAGACGGGCCCGAGGAGUGCGAUACAAGACGGGCCCGAGGAGUGACCGGUACAAGACGGGCCCGAGGAGUGUCACACAAGACGGGCCCGAGGAGUGUUUUUUGAAUUC

rno-miR-483-3p

Forward primer

GCTGACTCACTCCTCCCCTC

Reverse primer

TATGGTTGTTCACGACTCCTTCAC

U6 (rat)

Forward primer

ATTGGAACGATACAGAGAAGATT

Reverse primer

GGAACGCTTCACGAATTTG

SRF (human)

Forward primer

GAGATCGGTATGGTGGTCGG

 

Reverse primer

GTCAGCGTGGACAGCTCATA

SRF siRNA (human)

Sense

GCAUCAUGAAGAAGGCCUAUU

Anti-sense

UAGGCCUUCUUCAUGAUGCUU

β-actin (human)

Forward primer

ACATCCGCAAAGACCTGTAC

Reverse primer

GCCATGCCAATCTCATCTTG

SRF (rat)

Forward primer

ACCAGCTTCACTCTCATGCC

Reverse primer

TGCATGGGGACTAGGGTACA

β-actin (rat)

Forward primer

ACCCGCGAGTACAACCTTC

Reverse primer

ATGCCGTGTTCAATGGGGTA