Gene name | Primer usage | Sequence (5′–3′) |
---|---|---|
hsa-miR-199a-3p | RT primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACCTGCCC |
Forward primer | GGTGTCACTCCTCTCCTCC | |
Reverse primer | CAGTGCGTGTCGTGGA | |
hsa-let-7e-5p | RT primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAACTAT |
Forward primer | GGGGTGAGGTAGGAGGTTGT | |
Reverse primer | GTGCGTGTCGTGGAGTCG | |
hsa-miR-483-3p | RT primer | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACAAGACG |
Forward primer | GGTGTCACTCCTCTCCTCC | |
Reverse primer | CAGTGCGTGTCGTGGA | |
U6 (human) | RT primer | CGCTTCACGAATTTGCGTGTCAT |
Forward primer | GCTTCGGCAGCACATATACTAAAAT | |
Reverse primer | CGCTTCACGAATTTGCGTGTCAT | |
hsa-miR-199a-3p | Agomir: sense | ACAGUAGUCUGCACAUUGGUUA |
Agomir: antisense | ACCAAUGUGCAGACUACUGUUU | |
Antagomir: sense | UAACCAAUGUGCAGACUACUGU | |
hsa-let-7e-5p | Agomir: sense | UGAGGUAGGAGGUUGUAUAGUU |
Agomir: antisense | CUAUACAACCUCCUACCUCAUU | |
Antagomir: sense | AACUAUACAACCUCCUACCUCA | |
hsa-miR-483-3p | Agomir: sense | UCACUCCUCUCCUCCCGUCUU |
Agomir: antisense | GACGGGAGGAGAGGAGUGAUU | |
Antagomir: sense | AAGACGGGAGGAGAGGAGUGA | |
rno-miR-483-3p | Overexpression | GGAUCCGCACUCCUCCCCUCCCGUCUUGUCUGAGAAACAAGACGGAAGGAAACGUCACUUUUUUGAAUUC |
rno-miR-483-3p sponge | Sponge | GGAUCCACAAGACGGGCCCGAGGAGUGCGAUACAAGACGGGCCCGAGGAGUGACCGGUACAAGACGGGCCCGAGGAGUGUCACACAAGACGGGCCCGAGGAGUGUUUUUUGAAUUC |
rno-miR-483-3p | Forward primer | GCTGACTCACTCCTCCCCTC |
Reverse primer | TATGGTTGTTCACGACTCCTTCAC | |
U6 (rat) | Forward primer | ATTGGAACGATACAGAGAAGATT |
Reverse primer | GGAACGCTTCACGAATTTG | |
SRF (human) | Forward primer | GAGATCGGTATGGTGGTCGG |
 | Reverse primer | GTCAGCGTGGACAGCTCATA |
SRF siRNA (human) | Sense | GCAUCAUGAAGAAGGCCUAUU |
Anti-sense | UAGGCCUUCUUCAUGAUGCUU | |
β-actin (human) | Forward primer | ACATCCGCAAAGACCTGTAC |
Reverse primer | GCCATGCCAATCTCATCTTG | |
SRF (rat) | Forward primer | ACCAGCTTCACTCTCATGCC |
Reverse primer | TGCATGGGGACTAGGGTACA | |
β-actin (rat) | Forward primer | ACCCGCGAGTACAACCTTC |
Reverse primer | ATGCCGTGTTCAATGGGGTA |