Fig. 5

PEBP inhibited ERK1/2 but enhanced MAPKp38, and JNK signaling. Phosphorylation of ERK1/2, MAPK p38, and JNK in 4T1 (a, d, g), MDAMB-231 (b, e, h), and MCF-7 (c, f, i) mammospheres after treatment with 150 μM GAE (gallic acid equivalent) of either polyphenol-enriched blueberry preparation (PEBP) or normal blueberry juice (NBJ) for 2 h. All values are means of 3 separated experiments ±SEM. Baseline represent the level of phosphorylation present in cells not exposed to the mammospheres forming medium. * Denotes statistical significance at p ≤ 0.05 vs. control at 2 h