Fig. 6

Comparison of tumor size in brain sections from vaccinated and mock-vaccinated mice. Animals from the two groups were sacrificed in pairs when any control mouse showed signs of discomfort. a shows corresponding sections of the brain (stained by H&E) from three pairs of mice for comparison. Tumors from mock-vaccinated mice (i, iii, v) occupied most of the right hemisphere. In contrast, tumors from vaccinated mice (ii, iv, vi) were much smaller. b shows the bar graph of tumor volumes for each of the seven pairs of mice. c shows the bar graph of the cumulative data; tumor volumes were significantly smaller in vaccinated mice compared to mock-vaccinated mice (p < 0.0031). Tumor volumes (in mm³) were estimated using image analysis (NIH Image software ImageJ 1.37v)