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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: A therapeutic cancer vaccine against GL261 murine glioma

Fig. 2

Bioluminescence Imaging (BLI). All mice received an intracranial injection of 2 × 104 GL261 cells/mouse. For BLI, mice were anesthetized in a chamber containing a mixture of isoflurane (Florane, Baxter) and injected i.p. with 150 μL of the bioluminescent substrate ReadyJect d-Luciferin. Mice were placed within the chamber of a Xenogen IVIS 200 Bioluminescence and Fluorescence Imaging System for up to 10 min and sets of serial images obtained. Eight mice were imaged 2 days post-implantation of GL261 cells and six of them (75 %) showed measurable luciferase signal within the brain. a shows six of the eight imaged mice, including mice #1 and #5 which showed no signal. On day four post-implantation, both mice #1 and #5 showed a positive signal within the brain (b)

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