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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Kinetics of human myeloid-derived suppressor cells after blood draw

Fig. 1

Kinetics of gMDSC and mMDSC levels. Frequency of gMDSC (a) and mMDSC (b) within PBMC after 2, 4, 6 h, overnight rest (ON) and freezing/thawing procedure (defrost). PBMC were stored in RPMI media after the ficoll separation. gMDSC were analysed in 24 patients with chronic viral infections and mMDSC in 17 patients with solid tumors and three HIV infected patients. There was no statistical significant difference in gMDSC frequencies over time (2, 4, 6 h, ON). Statistical significant reduction of gMDSC frequencies after freezing/thawing procedure (p = 0.0001; Mann–Whitney test). The difference in frequencies in mMDSC is not significant after 4 h whereas their numbers were significantly reduced after a 6 h and overnight rest (p = 0.0005 and p = 0.005, respectively; Mann–Whitney test). mMDSC were more stable towards freezing/thawing, however the difference reached statistical significance (p = 0.039; Mann–Whitney test)

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