Table 2 NanoPro1000 assay conditions
Target | Primary antibody, dilution | Secondary antibody | Gradient | Immobilization time (s) |
|---|---|---|---|---|
AKT | Anti-AKT (pan), 1:50 | Anti-rabbit-HRP | G2 pH 5‒8 (nested) | 80 |
ERK1/2 | Anti-ERK 1/2 (pan), ready to use | Anti-rabbit-HRP | G2 pH 5‒8 (nested) | 80 |
MEK1/2 | Anti-MEK1/2 (pan), 1:12.5 | Anti-mouse-HRP | G2 pH 5‒8 (nested) | 80 |
c-MET | Anti-c-MET (pan), 1:12.5 | Anti-rabbit-HRP | G2 pH 5‒8 (nested) | 100 |